PacBio Revio

The Revio system adds affordability, high throughput, and ease of use to a foundation of long reads, exceptional accuracy, and direct methylation detection.

With a high-density, 25 million ZMW SMRT Cell, up to 4 SMRT Cells in parallel, and 24-hour run times, the Revio system delivers 360 Gb of HiFi reads per day, equivalent to 1,300 human whole genomes per year. ​

HiFi sequencing provides small variants, structural variants, repeat expansions, methylation, and haplotype phasing from a single library and sequencing run. ​

Operating the Revio system is simple, thanks to a combined reagent and sample plate, rapid run setup, flexibility to queue runs while sequencing is in progress, and no external nitrogen supply. ​

Advanced algorithms on the Revio system deliver exceptional read accuracy plus methylation calling in every run. Its optimized file formats reduce data storage needs through quality value binning and smart read ordering.

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With the Revio system, you can achieve higher throughput to support large studies and develop novel applications. The Revio system can generate 1,300 human genome equivalents per year, 15× more than the Sequel IIe system, via:

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Exceptionally accurate, high-throughput long-read sequencing at scale is worth celebrating!

The MedTech Breakthrough Awards recognize the top companies, people, platforms and products in the health, fitness and medical technology industries today.

The Revio system simplifies the HiFi sequencing experience with fewer consumables optimized for easy tracking and minimal waste. With workflow improvements, loading consumables takes under a minute at the instrument.

The polymerase kit contains optimized reagents to bind polymerase to 24 SMRTbell libraries in preparation for sequencing.

One tray contains 4 high-density SMRT Cells, each with 25 million zero-mode waveguides.

The sequencing plate provides reagents for sequencing up to 4 Revio SMRT Cells and combines multiple parts from the Sequel IIe system: sequencing plate, sample plate, mixing plate, and SMRT Cell oil.

The Revio system gives you a vastly simplified experience with consumables and software designed for flexibility and convenience. Whether you’re a genome center or a small lab, you can enjoy:

Library prep for the Revio system supports manual and automated preps with SMRTbell prep kit 3.0.

Sequencing plates are linked automatically to run designs through an NFC tag, enabling runs to be set up in less than one minute on instrument.

The Revio work deck is isolated from the four sequencing stages, leaving the system available up to 20 hours per day for loading consumables for subsequent runs.

The Revio system includes powerful NVIDIA GPUs, which provide more than 20× the compute power of the Sequel IIe system, to deliver accurate, ready-to-use data directly from the instrument.​

The GPUs provide rapid turnaround time for basecalling and HiFi read generation, directly outputting HiFi reads in BAM format.

DeepConsensus uses advanced deep learning techniques to further extend the accuracy and yield of HiFi sequencing.

Standard runs include DNA methylation status, calculated with a deep learning algorithm that processes polymerase kinetics.

All fundamental processing steps are performed on instrument, including barcode demultiplexing.

The Revio system produces the same — and better — read length, quality, and variant calling performance that have made HiFi sequencing so celebrated.

HiFi reads are tens of kilobases long, providing the ability to resolve large variants and map to difficult regions of the genome.​

The Revio system provides reliable answers through exceptional accuracy, with 90% of bases ≥Q30 and median read accuracy ≥Q30.​

HiFi sequencing on the Revio system delivers high accuracy for all variants types, matching the precisionFDA-winning accuracy of the Sequel IIe system.1

“…the high-throughput capabilities of the Revio system will allow us to apply HiFi technology to a wider array of applications in agricultural biotechnology“

Nanobind DNA kits enable extraction of HMW gDNA from a variety of sample types.

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SMRTbell prep kit 3.0 supports manual and automated library prep for effortless SMRTbell library creation.

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The Revio system offers simultaneous acquisition of 4 SMRT Cells on a 24-hour run, with flexible run scheduling to fit your schedule.

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Advanced algorithms from Google Health and cutting-edge NVIDIA GPUs allow for on-board processing of sequencing data, all with reduced output file sizes.

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HiFi yield2, 6

HiFi reads enable a wide variety of applications with different coverage, read length, and accuracy requirements. Based on typical yields and coverage requirements, the performance for select popular applications is:

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When will the Revio system be available?

Now. The Revio system is available to order and has shipped to customers worldwide

What is the price per genome on the Revio system?

U.S. list price is $995 for sequencing reagents for one Revio SMRT Cell, which has an expected yield of 90 Gb, equivalent to a 30X human genome. Your local sales representative can provide detailed pricing in your currency.

Are SMRTbell libraries prepared for the Sequel IIe system compatible with Revio?

Yes. The same library prep protocols are available for the Sequel systems and the Revio system. SMRTbell libraries can be used across systems. The sequencing reagents — polymerase/binding kit, sequencing plate, and SMRT Cell tray — are specific to each system. Currently the Revio polymerase kit supports libraries ≥3 kb; support for all library sizes is coming soon.

Is the output file format the same between the Sequel IIe system and the Revio system?

Yes. The hifi_reads.bam format continues to adhere to the SAM specification. The Revio BAM format has minor differences – for data size efficiency – in base quality encoding and read sort order.

See it all at scale with the Revio system.

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